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Sequencing at Micropathology

Offering affordable, rapid-turnaround Sanger and Next Generation Sequencing.

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Sanger Sequencing

Sanger Sequencing is a method used to determine the sequence of nucleotides in a piece of DNA. We start by mixing your amplified DNA template with primers and a blend of standard and fluorescently tagged terminator nucleotides.

After heating to denature the DNA, the primers bind to the single-stranded template, allowing DNA polymerase to extend the sequence.

The inclusion of fluorescently tagged terminators causes random chain termination which creates a pool of DNA fragments of varying lengths. These fragments are then separated and analysed, revealing the DNA sequence as the fluorescent tags are detected.

Our Application

Pathogen Detection

We use phylogenetic typing to find pathogens where traditional diagnostic tests cannot be applied.

Genotyping

We identify genetic variations and specific genotypes, providing essential clinical insights.

Drug Resistance

We pinpoint genetic mutations that lead to drug resistance, helping in the effective management medicine.

Next Generation Sequencing (NGS)

We use two Illumina MiSeq instruments for paired-end next-generation sequencing, where DNA is broken into short fragments and tagged with unique adaptors. These tagged fragments bind to a flow cell, where they are amplified to create clusters of DNA ready for sequencing.

The sequencing process involves adding fluorescently labelled nucleotides, which emit signals that are read and translated into DNA sequences by the MiSeq. This generates both forward and reverse reads, providing comprehensive data in fastq format.

Additionally, we utilize Oxford Nanopore technology for long-read sequencing, enhancing our research and diagnostic capabilities.

More Information

As well as customised research projects, we are developing diagnostics assays for pathogen detection and drug resistance screening using the MiSeq/ONT platforms. Get in touch to see if our sequencing services are appropriate for your project.

CONTACT US
Other Services
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Pathogen Detection

Rapid detection of Pathogens and Host Responses.
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Antibody/Antigen Detection

Rapid detection of specific antibodies in human and veterinary samples.
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Genetics

Genetic Profiling for human identification. Haemochromatosis, Prothrombin and Factor V Mutations
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Biomedical Research

We undertake grant-aided research with our colleagues in universities and in other research companies.