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Carbapenem Resistance Gene Detection Assay
06 Aug 2024

We are pleased to announce the launch of a probe based carbapenem resistance gene detection assay at Micropathology Ltd.

Carbapenems constitute some of the most effective and broadest-spectrum antibiotics available and are typically reserved for severe and multi-drug-resistant infections. Acquired carbapenemases are enzymes which inactivate carbapenems and most other β-lactam antibiotics, including penicillins and cephalosporins, and can result in infections with severely limited treatment options. At Micropathology, we have developed a probe based multiplex qPCR assay that is rapidly capable of detecting the ‘big five’ prominent carbapenemase resistance gene families including blaKPC, blaIMP-1, blaVIM, blaNDM-1 and blaOXA-48-like.
See below for genes that are detectable using our assay:


Carbapenemase Family

Variants

blaKPC

KPC-2-8, KPC-10-19, KPC-21-123, KPC-125-128, KPC-130-135, KPC138-148, KPC-151, KPC-153, KPC-155-157

blaNDM

NDM-1-31, NDM-33-50 (including NDM-16a and NDM-16b)

blaVIM

VIM-1-20, VIM-23-80, VIM 82-83, VIM-86

blaIMP

 IMP-1, IMP-3, IMP-6, IMP-10, IMP-25, IMP-30, IMP-34, IMP-40, IMP-42, IMP-52, IMP-55, IMP-60, IMP-61, IMP-66, IMP-70 IMP-76, IMP-77, IMP-78, IMP-79, IMP-80, IMP-88, IMP-97, IMP-98, 

blaOXA-48-like

OXA-48, OXA-162, OXA-163, OXA-181, OXA-199, OXA-204, OXA-232, OXA-244, OXA-245, OXA-247, OXA-252, OXA-370, OXA-405, OXA-416, OXA-438, OXA-439, OXA-484, OXA-505, OXA-514, OXA-515, OXA-517, OXA-519, OXA-538, OXA-546, OXA-547, OXA-566, OXA-567, OXA--788, OXA-793, OXA-833, OXA-894, OXA-918, OXA-920, OXA-922, OXA-923, OXA-924, OXA-929, OXA-933, OXA-934, OXA-1038, OXA-1039, OXA-1055, OXA-1119, OXA-1146, OXA-1181

 

Assay sensitivity for all five targets is approximately 200copies/mL and the current test turnaround time is 3 days from sample receipt. The assay is validated for various sample types including faecal swabs, faeces, tissue, cultures, EDTA WB, eye fluid, joint fluids and urine.

If you have any questions or require further information about the carbapenem resistance gene detection assay, please email info@micropathology.com or phone 02476 323222.