The quality of sequence data obtained from a GS Junior sequencing run directly reflects the quality of the starting material. Template DNA should meet the following minimum criteria:
DNA concentration should be assessed using a fluorometric technique.
Amplified DNA may be submitted but Roche Fusion primers* must have been used (with MIDs if multiplexing) and the post-amplification sample must be purified of all short products. If you are considering this approach, please contact us to discuss your submission.
*Fusion primer sequences:
5'–CGT ATC GCC TCC CTC GCG CCA TCAG–MID–template specific sequence–3'
5'–CTA TGC GCC TTG CCA GCC CGC TCAG–MID–template specific sequence–3'